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The protein concentration was measured using the bicinchoninic acid protein assay kit (Thermo Scientific, Waltham, MA)

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Other copper compounds tested in the same study did produce inflammation

The results demonstrated that the supplementation of the culture medium with 20 M leonurine (Leo20) resulted in a significant enhancement of blastocyst development, as evidenced by increased numbers of ICM, TE, and TCC in embryos when compared to the control group

Once inside the cell or at the receptor level, GHK-Cu works through several interconnected pathways: 1