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Samples were reduced with dithiothreitol at 10 mM for 30 minutes at 56C and alkylated with iodoacetamide at 30 mM for 45 minutes at room temperature in the dark

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The synthetic polymers used in this process serve two main purposes: they first dissolve the cell membrane, similar to a detergent, and then, using natural cellular phospholipids, they form a nanodisc structure around the membrane proteins