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All cell lines were kept in DMEM (PAN) with 10% FCS and 1% penicillin/streptomycin

Starting doses begin at 0.25 mg and may reach 14 mg daily, compared to injectable doses that start at 0.25 mg weekly and cap at 2.4 mg weekly

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(2017) observed that TMAO treatment promoted monocyte adhesion through upregulation of vascular cell adhesion molecule-1 (VCAM-1) but not the other adhesion molecules (i.e., intercellular adhesion molecule-1 (ICAM-1) and E-selectin) expression in human umbilical vein endothelial cells in vitro