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The crude product was purified by a silica gel column chromatography using hexane and dichloromethane mixture (10:1 v/v) as eluent and obtained as yellow powder in 74% yield

Studies have demonstrated that ethosomal gels had higher transdermal flow and skin deposition than normal gels, proving their usefulness in delivering medicinal medications such as Spironolactone for acne treatment [130]

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However, when HEK293 cells had their fsp1 gene knocked out (FSP1 KO), the residual VKR activity was in fact sensitive to warfarin inhibition to a similar inhibition potency as that of warfarin inhibition of VKOR reducing K epo (Fig