Oocyte Maturation Rate: It is generally related to nuclear maturity, being defined as the proportion of oocytes at MII stage ( Normal fertilization: Defined as the appearance of two protonuclei and two polar bodies at 16~18h after insemination ( Day three (D3) top-quality embryos: They are generally defined as those originating from normally fertilized oocytes with a cell count of 79 on day three after fertilization, the fragmentation rate is less than 10%, and there are no multinucleated embryos ( Blastocyst formation rate: Defined as the proportion of 2PN zygotes (not just of cleaved zygotes) which are at the blastocyst stage at Day 5 (116 2 h post-insemination) ( Usable blastocyst rate: This term denotes the combined count of transferred and frozen blastocysts within a single oocyte retrieval cycle
A vertical scratch was made in the center of each well using a sterile 200 L pipette tip
Product Information Product Name: Glutathione Form: Lyophilized synthetic peptide powder Peptide Length: 3 amino acids Composition: Glutamic acid, cysteine, and glycine Purity: At or above 98% (see batch-specific Certificate of Analysis) Testing: Batch-specific third-party HPLC, Mass Spectrometry (MS), and Endotoxin (LAL) testing available Intended Use: Research use only Storage: Store according to recommended laboratory handling procedures
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After centrifugation for 20 min at 931 g (room temperature), the cell layer at the interface was collected, washed and resuspended in PBS
