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s for the Met, Tip, Cys , and Tyr analogs were respectively determined to be: 1.375xl0 "07 M , 3.372x lO "09 M, 1.330xlO ",0 M, and 2.426x l 0 "10 M

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Immediately following addition of the substrate, fluorescence was monitored using a M5 Spectramax plate reader using SoftMax Pro 5 at 37 C, excitation at 529 nm and emission at 600 nm, with a 590 nm cut-off filter