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Protein samples were mixed with Laemmli sample buffer, boiled at 95C for 5 minutes, and separated on a 12% SDS-polyacrylamide gel

We then counted the number of DNA fragments recovered per gene and used this as a reference library for subsequent BarSeq experiments to assay depletion of strains with mutations in specific genes upon phage infection

Approximately 1.1 billion compounds were screened via this platform, and multiple candidate compounds with geroprotective properties that demonstrate structural novelty and target specificity were successfully identified

It is possible to be in the reference range and be still low in Vitamin B12 at a cellular level, especially if you are supplementing with B12