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DOI: 10.1080/19390211.2017.1409852
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After seeding at 37C, cells were treated with Lysosome inhibitors (Chloroquine 50g/ml and /or Bafilomycin 400nM, Sigma-Aldrich) or vehicle alone (DMSO) diluted in DMEM medium for 4h (37C) and stained with LysoTracker DND-99 (Invitrogen)
Cells were seeded in advanced DMEM/F-12 (Thermofisher) supplemented with R-Spondin (10% v/v) and Noggin (10% v/v) conditioned media, B-27 supplement (Thermofisher) (1X), nicotinamide (10 mM) and FBS (1%)
In white fat vasculature, these proteins work together with CD36, a fatty acid transporter, to shuttle long-chain fatty acids from the bloodstream into adipocytes
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