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Thus, over the years, research has been conducted into the possible link between an even moderate rise in homocysteine levels and cardiovascular risk and mortality, with conflicting results [6,7]

Hormone measurements Commercially available enzyme-linked immunosorbent assay (ELISA) kits were used to determine the serum concentration of leptin (Crystal Chem), T4 (Calbiotech), testosterone (Calbiotech), corticosterone (Arbor Assays), GH (Millipore), IGF-1 (R&D Systems) and prolactin (Sigma)

Plasma levels drop from approximately 200ng/ml at age 20 to 80ng/ml by age 60

The food and drinking water were sterilized

Telomere and Genetic Stability Research