After labeling, cells were fixed, permeabilized, and treated with 2 N HCl at room temperature for 30 minutes to denature the DNA, then neutralized with 0.1 M sodium borate (pH 8.5)
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Glutathione tackles this at a biological level by: Inhibiting tyrosinase activity to slow down melanin production Shifting melanogenesis from eumelanin (darker) to pheomelanin (lighter) Acting as an antioxidant to reduce the oxidative stress that triggers hyperpigmentation A 2025 systematic review in the International Journal of Dermatology confirms that oral glutathione at doses of 250 mg twice daily and 500 mg once daily showed a significant reduction in melanin index compared to placebo, particularly in sun-exposed areas like the face and wrists