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As far as NAD+ precursors potential to slow aging goes, a year-long study with adults aged 54 to 84 showed that NMN supplementation was associated with significantly reduced acceleration of epigenetic agea measure of how fast cells are aging based on chemical modifications to DNA

It also modulates the activity of matrix metalloproteinases (MMPs) and their inhibitors, supporting balanced collagen remodeling rather than excessive breakdown or fibrosis

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Proteins were transferred to PVDF membranes (Thermofisher) using a Power BlotterSemi-dry Transfer System (Thermofisher) and incubated overnight with IFN- (E3V1X) Rabbit mAb (Cell Signaling, #98139S), Galectin-1/LGALS1 (D608T) Rabbit mAb (Cell Signaling, #12936S, Granzyme B (E5V2L) Rabbit mAb (Cell signaling, #44153) and SHMT2 (E7F4Q) Rabbit mAb (Cell signaling, #3344), vinculin Mouse mAb (Sigma-aldrich, #V9131), actin (clone C4) Mouse mAb (MP biomedicals, #691001) antibodies diluted in 5% skimmed milk in Tris-Buffered NaCl Solution with Tween 20 (TBST)