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Biphasic half-life [edit] Many drugs show a biphasic decline in plasma concentration after a dose: a steep distribution phase as drug leaves the central compartment for tissues ( phase), followed by a shallower elimination phase as drug is cleared ( phase)

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For each gradient, about 30 fractions (400 l) were collected from the top and the radioactivity was quantified by liquid scintillation counting (Perkin Elmer)