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A portion of the sample was reduced with 100 mM DTT (final concentration) to remove the BioGEE label

Following a 1 h incubation with the appropriate secondary antibody, Clarity Western ECL Substrate was applied to the membrane and bands were visualized using a Biorad Chemidoc MP to first pixel saturation and densitometry performed using Image Lab (Biorad, Hercules, CA)

Acknowledgment: This guideline was produced in collaboration with the Practice Guidelines Committee of the American Association for the Study of Liver Diseases and the Practice Parameters Committee of the American College of Gastroenterology

2011 Oct 26

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