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Likewise, the addition of CB-839 to venetoclax, AZD-5991, or ibrutinib along with cytokine stimulation did not increase the apoptosis rate (P = 0.67, P = 0.71, and P = 0.85, respectively

Total glutathione lysis reagent or oxidized glutathione lysis reagent (25 l) was added to respective wells containing worm acid supernatants and the plate was shaken for 5 min

doi: 10.1016/j.envexpbot.2018.05.003 58 FoyerC

Notably, similar inhibitions were observed both with the wild type enzyme and its cysteine mutants, suggesting that thiol conjugation is dispensable for GST inactivation by auronofin in contrast to other described targets 103

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