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Let's start with the basics, so you know exactly what you're working with

Extended Half-Life: Providing a prolonged experimental window for chronic, long-term animal longevity and weight loss trials without inducing receptor down-regulation artifacts

TB-500 Research Foundation Landmark research published in Nature by Bock-Marquette et al

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the resulting almost clear solution is applied on a column of AmberliteTMIRl20H ion-exchange resin (Rohm & Haas), and then on Resindion SepabeadsTMSP207 resin (Mitsubishi), and finally concentrated by nanofiltration, as described in paragraphs [0060] and [0061] of said patent