In addition to the cell culture media described above, strial explants can also be maintained in the following media: MEM with Earle's salts, 15 mM HEPES buffer, 2 mM L-glutamine, 10 mg/L MEM non-essential amino acids, 2.5 mg/L fungizone, 100 units/ml penicillin, 100 mg/L streptomycin, 400 g/L hydrocortisone, 5 mg/L transferrin, 2 nM triiodothyronine, 5 mg/L insulin, 100 M cholera toxin, 10 g/L epidermal growth factor, and 10% fetal calf serum
10.1371/journal.pone.0142049 55 KongsupholP.MukdaS.NopparatC.VillarroelA.GovitrapongP
A hallmark of ferroptosis is iron-dependent lipid peroxidation, which could be inhibited by the key ferroptosis regulator Gpx4, free radical trapping antioxidants and ferroptosis-specific inhibitors 501
The glucagonostatic and insulinotropic effects of glucagon-like peptide 1 contribute equally to its glucose-lowering action
and 75 L of the cell homogenate