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Here are some things to look for after the five minutes is up

Always consult primary peer-reviewed literature (PubMed, Google Scholar) before designing a study, and validate dose-ranges, vehicle compatibility, and analytical methodology against the original published methods

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10.1046/j.1432-1327.2001.02221.x 83 MatsumotoH.RogiT.YamashiroK.KodamaS.TsuruokaN.HattoriA.et al (2000)