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Regional anesthesia or monitored sedation might permit greater flexibility, but record the anesthesia regimen and medication directions in the pre-operative checklist

Improper storage degrades the peptide, reducing its effectiveness with each passing day

Sagittal 4 m paraffin sections of the brain were mounted on slides, which were rehydrated and incubated in a citrate buffer at 95 for 30 minutes for antigen retrieval