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The affinity of PFK-1 for fructose-6-phosphate (F6P) is mediated by its potent activator (i.e., fructose 2,6-bisphosphate (F2,6P2)), which converts F6P to fructose-1,6-bisphosphate (F1,6P2)

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Additionally, stress-inducing agents such as tunicamycin, thapsigargin and histidinol have been shown to modulate CHAC1 regulation, indicating its involvement not only during ER stress but also in the context of the ISR ( CHAC1 promoter is the primary mechanism for the transcriptional upregulation of the gene