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Purified membranes were resuspended in 40 mL low salt buffer and incubated with 100 M PF-06372222, 2 mg mL 1 iodoacetamide, and EDTA-free protease inhibitor cocktail tablet for 1 h at 4 C

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Medical review: Schedule a pre-conception consultation with your GP to review overall health, optimise management of comorbidities (diabetes, hypertension), and ensure any other medications are pregnancy-safe

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A paper published in May 2022 reviewed a case of injection site reaction following subcutaneous injection of methotrexate