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All steps in the preparation of the enzyme extract were carried out at 4 C

[14] Whether those effects have been independently replicated across groups to the standard the word reproducible implies is a separate question, and one this article is deliberately not asserting

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That is, the p Ka of GSSH is 6.9, which is two orders of magnitude lower than that of GSH, which is 8.9 ( Ka implies that supersulfides have a greater tendency to deprotonate and form anionic species under physiological conditions, thereby increasing their reactivity with electrophilic molecules