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Recent investigations have revealed that ferroptosis involves alterations in iron metabolism regulatory proteins (e.g., GPX4, TfR1), accumulation of lipid peroxidation products, and dysregulation of antioxidant systems, particularly the nuclear factor E2-related factor 2 (Nrf2)/glutathione peroxidase 4 (GPX4) pathway (8, 9)

Further, the combined use of Sph_12-38 and SHS resulted in a cure rate of 100% for rabbit ear acne treatment in vivo for two weeks, while the one induced by other groups was 40%, 0% and 0% for SHS alone, Sph_12-38 alone and control group, respectively

If we aimed to look at the effects of the study agent in inflammatory lesions, a longer intervention period may be needed