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DEGs were identified using the DESeq2 R package, and results were visualized as a volcano plot using the ggplot2 R package ( Metabolic genes were defined based on a curated list 6,40

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The first step in decoding DILI is to classify the pattern of liver injury: Hepatocellular injury is characterized by marked elevations in alanine aminotransferase (ALT) or aspartate aminotransferase (AST), usually three times or more above the upper limit of normal (ULN)

Rotating injection sites prevents tissue irritation and ensures consistent absorption