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We don't have robust long-term data on: What happens to muscle mass and bone density over 5-10 years of use The impact on people who start these medications in their 20s or 30s Impact on fertility/reproductive outcomes Early data is showing that GLP-1 might help in PCOS, but what about long-term hormonal regulation

Healthy Fats in Small Amounts Fat is still part of a healthy diet, but portion size matters more on a GLP-1 medication because very rich meals can sometimes make nausea, reflux, or fullness worse

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Then cDNA was synthesized using SuperScript TM IV VILO TM Master Mix (Invitrogen, Waltham, MA, United States) and diluted 101 by DNase/RNase-Free Distilled Water (Invitrogen)

Measurement of interleukin (IL)-1 and tumor necrosis factor- (TNF-) The inflammatory cytokines, TNF-, and IL-1 were quantified using ELISA kits (430915 and 432615