After a 20-min incubation with immunol staining blocking buffer (Beyotime, Shanghai, China), cells were continuously incubated with mouse anti-SMA (1:100, Abcam) and rabbit anti-CD133 (1:100, Abnova) 44 at 4 C overnight
Originally identified in relation to sleep architecture, DSIP has become a significant focus of research involving restorative sleep, circadian rhythm balance, and neuroendocrine activity
Certain muscle groups observed in murine models, including the rectus abdominis, psoas major , and paraspinal muscles, showed a more pronounced increase in muscle density and volume, as well as a reduction in intramuscular fat content, than those observed in controls
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