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The beads were then washed three times with 1 ml of RIPA buffer, followed by washes with 1 M KCl, 0.1 M Na 2 CO 2 and 2 M urea in 10 mM Tris-HCl (pH 8.0)

Consider both only when the research objective clearly benefits from addressing both superficial tissue remodeling and deeper structural recovery pathways