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Typical Assay Conditions: Aqueous buffer : dH20 or buffered solution Incubation : 24 hours at room temperature Analysis : LC-MS/MS after centrifugation Compound Requirements : 2 times 1 mg for each assay condition plus 1 mg for preparation of standards

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It has been determined that nuclear factor erythroid-2 (Nrf2)/heme oxygenase (HO-1) signal pathway plays a strong part in antioxidant stress and the overexpression of Nrf2 can dramatically reduce ischemic brain damage (149)