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By conducting KEGG metabolic pathway enrichment analysis on the differential metabolites of each group, it can be observed that a high-fat diet affects the biosynthesis of phenylalanine, tyrosine, and tryptophan, taurine and hypotaurine metabolism, phenylalanine metabolism, and arachidonic acid metabolism in mice

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NAC provides a precursor to glutathione, while vitamin B6 as P5P supports enzymatic activity involved in normal detoxification and cellular metabolism