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Description

To measure lysosomal dynamics, cells were incubated with 50nM LysoTrackerTM Deep Red (ThermoFisherScientific), and Hoechst 33342 in HBSS for 40 minutes and then imaged using Zeiss LSM 880 confocal microscope where the 405 nm, and 647 nm laser line were used to excite Hoechst 33342 and LysoTrackerTM Deep Red, respectively

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