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Gq/11 is believed to interact with PLC, which may then cleave a lipid molecule called phosphatidylinositol 4,5-bisphosphate (PIP2) into two secondary messengers: IP3 (Inositol trisphosphate) and DAG (Diacylglycerol)

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The plates were subsequently washed and incubated with biotin-labeled anti-IgG mAb (Mabtech, Sweden) and horseradish peroxidase-conjugated streptavidin (Mabtech, Nacka Strand, Sweden)

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