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Cell lines After 24 h-treatment in DMSO alone or supplemented with 5 M GW4064 12 , GLUTag and NCI-H716 cells were starved for 30 min in glucose-free Krebs buffer consisting of 120 mmol/l NaCl, 5 mmol/l KCl, 2.2 mmol/l NaHCO 3 , 1 mmol/l MgCl 2 , 2 mmol/l CaCl 2 and 20 mmol/l Hepes, supplemented with 0.2% bovine serum albumin (BSA) (pH = 7.27.4)

RAW 264.7 cells were activated with proinflammatory stimuli to induce increased intracellular ROS levels, followed by incubation with various formulations

So that became the springboard for all the drugs that people are on for cardiovascular disease now