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Anti-human GAPDH antibody (Abcam) (Supplementary Data 5) was used as the primary antibody and nuclei were counterstained with haematoxylin

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In research models, this may include: Food intake behavior Meal-size signaling Satiety response after nutrient intake Delayed gastric emptying models Brain-based appetite regulation Hormonal feedback related to energy availability This makes cagrilintide useful for studying how peptide signals affect both biological function and behavioral outcomes related to food intake

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