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Cells were washed with DPBS and lysed on ice with 100 l RIPA-Puffer (ThermoFisher Scientific) supplemented with protease (Roche Diagnostics) and phosphatase (Roche Diagnostics) inhibitors

No significant modification in BMD and no differences between groups in RM could be due to the relatively short duration of the RT intervention

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However, because it is a stimulant, it is not recommended for patients with a history of substance abuse