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High pH fractionation- TMT labeled, combined, and dried samples were resuspended in 300 L of Buffer A (5% v/v MeCN, 95% v/v H 2 O, 0.1% v/v formic acid) and fractionated by centrifugation using a peptide desalting column (Pierce, #89852)

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71 The SAPIR depends critically on an FDA-approved finished-dosage manufacturing network positioned to rapidly convert APIs into labeled medicineswith all regulatory approvals already in place

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