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Cerebrospinal fluid samples were thawed then 150 L immediately mixed with 150 L of methanol/water 50/50, thoroughly mixed on a vortex mixer, incubated in an ultrasonic bath for 5 min and incubated on ice for 20 min

SIPS is a phenomenon characterized by the irreversible cessation of the division of normal cells even in presence of nutritional and mitogenic factors 27

This study also received funding from Cooperation Projects of Sichuan Credit Pharmaceutical CO., Ltd

It creates a firm barrier in the skin structure and promotes the differentiation of keratin-forming cells, creating a protective barrier in the stratum corneum to maintain skin hydration, making dry skin moist

Allow the alcohol to evaporate completely before proceeding to prevent chemical interference with the peptide