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Immunofluorescence staining of brain tissue examined the expression of tumor necrotic factor-alpha (TNF-, pro-inflammatory cytokine), glial fibrillary acidic protein (GFAP, a marker of activated astrocytes), sequestosome 1 (p62, marker of autophagic activity), voltage-dependent anion channel 1 (VDAC1, marker of mitochondrial stress), and NADPH Oxidase 4 (NOX4, marker of mitochondrial stress)

Response: In response to the comment regarding section 508 compliance for Online delivery, we emphasize that the application of section 508 of the Rehabilitation Act, which requires Federal agencies to make their electronic and information technology accessible to people with disabilities, is unaffected

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