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After twice washing with PBS, the nuclei were counterstained with 10 g/ml DAPI (4,6-diamidino-2-phenylindole) (C0060, Solarbio) for 10 min at 37C

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Microbiome differences were greater between the two groups switched from WD to LD (i.e., WD/LD-V and WD/LD-T) and the two diet control groups (i.e., LD/LD-V and WD/WD-V) compared to the microbiome differences observed between the lean (LD/LD-V) and obese (WD/WD-V) control groups

[41] confirm that dietary supplementation with lipoic acid decreased both circulating leptin, and adipose tissue leptin mRNA in rats, while Aslfalah et al