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These strategies can restart metabolic activity when conventional approaches stall

Metformin is a biguanide that primarily reduces hepatic glucose production and improves insulin sensitivity in peripheral tissues without causing hypoglycaemia

Evans-blue vascular permeability assay To assess vascular permeability, animals (n=9-9) received 50 mg/kg Evans blue (Reanal, Hungary, dissolved in physiological saline at a concentration of 2.5%) via a femoral venous catheter

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Cell were washed and placed in normal Ringers buffer (160 mM NaCl, 4.5 mM KCl, 2 mM CaCl 2 , 1 mM MgCl 2 , 10 mM HEPES, pH 7.4) or Low Ca 2+ Ringers where CaCl 2 was omitted and 1 mM Ethylenediaminetetraacetic acid (EDTA) added