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Digoxin at a concentration of 5 mg/kg injected in mice with collagen-induced arthritis, bufatolin at a concentration of 100 g/kg injected in mice with induced Chagrin syndrome and gamabufatolin (8 ng/mL, 19 nM) in vitro show similar effects, including reduction of T-helper cells expressing IL-17 (Th17) and polarization and decreased production of key pro-inflammatory cytokines IL-1, IL-6, TNF- and IL-21 by the inhibition of retinoic acid receptor-related orphan receptor thymus (RORt) translational activity (Huh et al., 2011