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10.1056/NEJM199205143262003 9
High-yield isolation of murine microglia by mild trypsinization
Substrate MUPs (Merck, Sigma-Aldrich) were added and incubated for 1 h and 30 min at room temperature in the dark, and fluorescence was measured at an excitation wavelength of 360 nm and an emission wavelength of 440 nm with a Varioskan LUX 3,020-956 (Thermo Fisher Scientific)
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The test protocol consisted of two consecutive 4-min submaximal bouts (40 and 80 W for female individuals, 50 and 100 W for male individuals) followed by stepwise workload increments (20 W/min for female individuals, and 25 W/min for male individuals) until volitional exhaustion
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