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The purified peptide 20GCGRG s Nb35 complex at a concentration of 1214 mg/mL was mixed with 100 M peptide 20 at 4 C and applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, Au 300 mesh) that were subsequently vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific)
Both wild type and homozygous knockout male and female rats were generated in-house to produce pure wild type and pure homozygous litters used in the experiments
(A-F) Representative images from the various indicated treatments are shown (G) Concentration response plots of GFP-LC3 change after quantitative image analysis are shown
The appetite suppression they produce is significant
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