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Intracellular biliverdin dynamics during ferroptosis

Both collected biofluids were centrifuged at a speed of 2300 g for 10 min at 4C, transferred supernatant into a 1.5 ml cryopreservation tube, and stored at 80C until metabolite extraction ( Sample preparations for plasma, urine, hair, and tissue All samples were processed with a standard operating procedure.150 L aliquots of thawed plasma or urine were mixed with three internal standards (IS) [20 L of d4-alanine (Sigma, USA, 10 mM), d5-phenylalanine (Sigma, USA, 10 mM), and d5-tryptophan (Sigma, USA, 10 mM)]