Then, 15 g of protein was mixed with NativePAGE sample buffer (Invitrogen), Coomassie G-250 and protease inhibitor, loaded into the lanes of a 312% Bis-Tris NativePAGE gel (Invitrogen) and run according to the manufacturers instructions
For instance, auranofin also targets or inhibits other proteins, including proteasome and proteasomal deubiquitinases in breast cancer cells [234, 238]
An SOD1 deficiency enhances lipid droplet accumulation in the fasted mouse liver by aborting lipophagy
doi:10.3748/wjg.v18.i22.2756[PubMed] 52
designed a Host Microbiota Interaction (HMI) module, that permits analysis of aerobic and anaerobic microbes, including complex living microbiome derived from a human intestinal microbial ecosystem (SHIME) reactor, where both microbes and human Caco-2 cells were co-cultured under an oxygen gradient (141)