The remaining 60 to 74% comes from fat
For serum samples, 75 L of chloroform:methanol extraction solvent (v:v = 1:2) was added to 20 L of mouse serum, with the exception of the serum from WT, Gclc L-KO, Nrf2 L-KO, and L-DKO mouse serum, for which 20 L of mouse serum was combined with 180 L of chloroform:methanol extraction solvent (v:v = 1:2) containing internal standards at the final concentrations: 5 nM D7-Sphinganine (Avanti Polar Lipids Inc., Cat# 860658), 12.5 nM D3-Deoxysphinganine (Avanti Polar Lipids Inc., Cat# 860474), and SPLASH LIPIDOMIX (1:1000, Avanti Polar Lipids Inc., Cat# 330707)
We were therefore not able to differentiate the relative contribution of each to the overall insulinotropic response
Contained: Johnson said "contained" means the fire is surrounded by barriers either fire lines built by crews or natural breaks like roads, lakes and ridges
Author contributions XQ: Conceptualization, Data curation, Formal Analysis, Methodology, Supervision, Writing original draft
