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Furthermore, in each case the average electrode position is about 1 cm proximal to the intended position, which would be anticipated if the measurement process triggered this amount of shortening

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They were cultured, respectively in Dulbecco's Modified Eagle Medium, Iscove's Modified Dulbecco's Medium, and Roswell Park Memorial Institute 1640 medium (Hyclone) with 10% fetal bovine serum (Gibco) under incubation at 37C with 5% carbon dioxide

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This decreases delayed gastric emptying and increases aspiration risk with general anesthesia