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The membrane was then incubated in substrate equilibration buffer for 15 min and treated with a chemiluminescent substrate for 5 min

Solid-Phase Peptide Synthesis (SPPS) How SPPS works, Fmoc vs Boc chemistry, and modern synthesis advances

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[DOI] [PMC free article] [PubMed] [Google Scholar] 10.Song H, Ekheden IG, Zheng Z, et al

Beyond these defining features, a growing body of clinical and biochemical evidence indicates that PCOS is frequently accompanied by chronic low-grade inflammation, metabolic stress, and increased cardiometabolic risk [3, 4]