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significant covariates were retained in the final model analysis

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Subsequently, the ejaculates of Holstein bulls were pooled, split into four equal groups, and diluted with a Tris extender buffer supplemented with basic extender (negative control group, NC group), 2 g/ml SeNPs (SeNPs group), 4 mM GSH (GSH group), and 4 mM GSH plus 2 g/ml SeNPs (GSH + SeNPs group)

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