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Since the Slutske scale is technically not a validated way to assess hangover, a subset of five valid items from that scale were used to assess the effect of NAC on hangovers 14

Therapeutic applications of bilirubin and biliverdin in transplantation

Mostly animal and limited human aging studies

Sphincter pressures increased significantly compared to untreated controls

The relative abundances of Clostridium subcluster 4 of bacteria in the fecal samples were evaluated using quantitative PCR, which was performed using SYBR Green (Takara Bio, Kusatsu, Shiga, Japan) and an Mx3000P real-time PCR system (Agilent Technologies Japan Ltd., Tokyo, Japan), as reported previously [9]