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The landmark LEADER (Liraglutide Effect and Action in Diabetes: Evaluation of Cardiovascular Outcome Results) trial, which enrolled 9340 individuals with T2D at high CV risk, demonstrated that liraglutide significantly reduced the incidence of major adverse cardiovascular events (MACE) compared with placebo over a median follow-up of 3.8 years (Hazard Ratio [HR]: 0.87

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A vibratome (Leica VT1200S, Leica Biosystems) was used to obtain 250 m thin acute brain slices containing Arc, identified by well-known landmarks

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