Each compound is synthesized to exceed 99% purity and validated through advanced testing methods, including HPLC and mass spectrometry
Primary antibodies against SLC7A11 (Cell Signaling Technology, 12691, 1:500 dilution), iNOS (BD Biosciences, 610329, 1:500 dilution), 15LO1 (Abnova, H00000246-D01P, 1:1000 dilution), GPX4 (Abcam, 125066, 1:1000 dilution), POSTN (Santa Cruz Biotechnology, 67233, 1:500), and CCL26 (R&D Systems, AF653, 1:500 dilution) were used
H&E staining revealed that the atherosclerotic lesion areas in the aortic root cross-sections were significantly reduced in the HCD + ALC group compared with the HCD group (Figures 5D,E)
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Reinke SN, Naz S, Chaleckis R, Gallart-Ayala H, Kolmert J, Kermani NZ, et al